PrimePCR™ SYBR® Green Assay: MT4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050208

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MT4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MT4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042946
Assay Design:   exonic
Chromosome Location:   16:56602776-56602867question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000566692 ENST00000381423 ENST00000427980 ENST00000355305 ENST00000367731 ENST00000485254 ENST00000358155 ENST00000216122 ENST00000379284 ENST00000379287 ENST00000252482 ENST00000413988 ENST00000329099 ENST00000245816 ENST00000596149 ENST00000596605 ENST00000265318 ENST00000219162

Gene Information

Description Not Available

Gene Symbol:   MT4
Gene Name:   metallothionein 4
Aliases:   MT-4, MT-IV, MTIV
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000102891
Entrez:   84560
Chromosome Mapping:   16q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.070000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download