PrimePCR™ SYBR® Green Assay: ATP1B4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050150

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP1B4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP1B4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042888
Assay Design:   exonic
Chromosome Location:   X:119505047-119509336question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000218008 ENST00000361319 ENST00000539306

Gene Information

This gene has been found in all vertebrate genomes sequenced to date. However this gene has undergone a change in function in placental mammals compared to other species. Specifically in fish avian and amphibian species this gene encodes plasma membrane-bound beta-subunits of NaK-ATPase. In placental mammals the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2010]

Gene Symbol:   ATP1B4
Gene Name:   ATPase, Na+/K+ transporting, beta 4 polypeptide
Aliases:   Not Available
RefSeq:   NC_000023.10 NT_011786.16 NG_016773.1
Ensembl:   ENSG00000101892
Entrez:   23439
Chromosome Mapping:   Xq24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download