PrimePCR™ SYBR® Green Assay: GTPBP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050017

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042755
Assay Design:   exonic
Chromosome Location:   22:39102150-39111928question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000470836 ENST00000488787 ENST00000484657 ENST00000216044

Gene Information

This gene is upregulated by interferon-gamma and encodes a protein that is a member of the AGP11/GTPBP1 family of GTP-binding proteins. A structurally similar protein has been found in mouse where disruption of the gene for that protein had no observable phenotype. [provided by RefSeq Jul 2008]

Gene Symbol:   GTPBP1
Gene Name:   GTP binding protein 1
Aliases:   GP-1, GP1, HSPC018, MGC20069
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100226
Entrez:   9567
Chromosome Mapping:   22q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download