PrimePCR™ SYBR® Green Assay: CDC14B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049731

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CDC14B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CDC14B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042469
Assay Design:   exonic
Chromosome Location:   9:99285545-99285656question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000375241 ENST00000375240 ENST00000375242 ENST00000463569 ENST00000265659 ENST00000375236 ENST00000520309 ENST00000313581 ENST00000431575

Gene Information

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14 a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53 and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC14B
Gene Name:   CDC14 cell division cycle 14 homolog B (S. cerevisiae)
Aliases:   CDC14B3, Cdc14B1, Cdc14B2, hCDC14B
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000081377
Entrez:   8555
Chromosome Mapping:   9q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.780000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download