PrimePCR™ SYBR® Green Assay: MYH7B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049689

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MYH7B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MYH7B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042427
Assay Design:   exonic
Chromosome Location:   20:33590091-33590207question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000262873 ENST00000446156 ENST00000453028 ENST00000435272 ENST00000433934 ENST00000456649

Gene Information

The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain which catalyzes ATP hydrolysis and interacts with actin and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found but the full-length nature of these variants is not determined. [provided by RefSeq Mar 2010]

Gene Symbol:   MYH7B
Gene Name:   myosin, heavy chain 7B, cardiac muscle, beta
Aliases:   MGC96928, MGC96940, MHC14, MYH14
RefSeq:   NC_000020.10 NG_016984.1 NT_011362.10
Ensembl:   ENSG00000078814
Entrez:   57644
Chromosome Mapping:   20q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.260000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download