PrimePCR™ SYBR® Green Assay: SMARCE1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049598

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SMARCE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SMARCE1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042335
Assay Design:   exonic
Chromosome Location:   17:38792670-38792776question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000311181 ENST00000590714 ENST00000348513 ENST00000431889 ENST00000377808 ENST00000578044 ENST00000580419 ENST00000400122 ENST00000478349 ENST00000580654 ENST00000577721 ENST00000582955 ENST00000544009

Gene Information

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein either alone or when in the SWI/SNF complex can bind to 4-way junction DNA which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins this protein has no yeast counterpart. [provided by RefSeq Jul 2008]

Gene Symbol:   SMARCE1
Gene Name:   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Aliases:   BAF57, FLJ35648
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000073584
Entrez:   6605
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.950000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download