PrimePCR™ SYBR® Green Assay: MGAT4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best negative control; Same primer pair as used in probe assay qHsaCEP0049567

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MGAT4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MGAT4A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042304
Assay Design:   exonic
Chromosome Location:   2:99253054-99256405question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000264968 ENST00000409391 ENST00000414521 ENST00000393487

Gene Information

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein in addition to the related isoenzyme B catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-14 linkage to the Man-alpha-13-Man-beta-14-GlcNAc arm of R-Man-alpha-16(GlcNAc-beta-12-Man-alpha-13)Man-beta-14-GlcNAc-beta-14-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins oncogenesis and differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:   MGAT4A
Gene Name:   mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
Aliases:   GNT-IV, GNT-IVA
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000071073
Entrez:   11320
Chromosome Mapping:   2q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.980000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download