PrimePCR™ SYBR® Green Assay: PDZD4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049528

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: PDZD4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PDZD4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042265
Assay Design:   exonic
Chromosome Location:   X:153070234-153070337question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000419587 ENST00000307768 ENST00000355703 ENST00000449592 ENST00000374796 ENST00000359003 ENST00000567078 ENST00000347162 ENST00000453940 ENST00000259708 ENST00000479388 ENST00000394056 ENST00000394058 ENST00000309469 ENST00000164640 ENST00000544474 ENST00000393758 ENST00000252242 ENST00000368777 ENST00000276185 ENST00000296318 ENST00000320057 ENST00000463523 ENST00000427856 ENST00000595215 ENST00000595959 ENST00000601507

Gene Information

Description Not Available

Gene Symbol:   PDZD4
Gene Name:   PDZ domain containing 4
Aliases:   FLJ34125, KIAA1444, LU1, PDZK4, PDZRN4L
RefSeq:   NC_000023.10 NT_167198.1
Ensembl:   ENSG00000067840
Entrez:   57595
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.600000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download