PrimePCR™ SYBR® Green Assay: ST3GAL6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049468

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ST3GAL6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ST3GAL6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042205
Assay Design:   exonic
Chromosome Location:   3:98489794-98492776question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000483910 ENST00000460774 ENST00000497008 ENST00000486334 ENST00000468553 ENST00000485391 ENST00000492254 ENST00000477574 ENST00000485145 ENST00000394162 ENST00000265261

Gene Information

Sialyltransferases such as ST3GAL6 catalyze the transfer of sialic acid from cytidine 5-prime monophospho-N-acetylneuraminic acid (CMP-NeuAc) to terminal positions of glycoprotein and glycolipid carbohydrate groups. Terminal NeuAc residues are key determinants of carbohydrate structures such as the sialyl-Lewis X determinants and are widely distributed in many cell types.[supplied by OMIM Mar 2008]

Gene Symbol:   ST3GAL6
Gene Name:   ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Aliases:   SIAT10, ST3GALVI
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000064225
Entrez:   10402
Chromosome Mapping:   3q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.690000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download