PrimePCR™ SYBR® Green Assay: MED29, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049461

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MED29, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MED29, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042198
Assay Design:   exonic
Chromosome Location:   19:39888209-39888298question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000596297 ENST00000594368 ENST00000599213 ENST00000315588 ENST00000324932 ENST00000429801 ENST00000381176 ENST00000327492 ENST00000523300 ENST00000443491 ENST00000517600 ENST00000523782 ENST00000523623 ENST00000520174 ENST00000517804 ENST00000265800 ENST00000517418 ENST00000523266 ENST00000519907 ENST00000381470 ENST00000432128 ENST00000358242 ENST00000415253 ENST00000535622 ENST00000382105 ENST00000373755 ENST00000373754 ENST00000340587 ENST00000394319 ENST00000541397 ENST00000541364 ENST

Gene Information

MED29 is a subunit of the Mediator complex a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators RNA polymerase II (see MIM 180660) and general initiation factors (Sato et al. 2003 [PubMed 14576168]).[supplied by OMIM Aug 2009]

Gene Symbol:   MED29
Gene Name:   mediator complex subunit 29
Aliases:   DKFZp434H247, IXL
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000063322
Entrez:   55588
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.270000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download