PrimePCR™ SYBR® Green Assay: ELMO2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049457

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042194
Assay Design:   exonic
Chromosome Location:   20:45000487-45000578question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000290246 ENST00000372176 ENST00000452857 ENST00000352077 ENST00000396391 ENST00000439931 ENST00000445496 ENST00000454865

Gene Information

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   ELMO2
Gene Name:   engulfment and cell motility 2
Aliases:   CED-12, CED12, ELMO-2, FLJ11656, KIAA1834
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000062598
Entrez:   63916
UniGene:   Hs.210469
Chromosome Mapping:   20q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.930000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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