PrimePCR™ SYBR® Green Assay: LMO3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049375

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LMO3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LMO3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042112
Assay Design:   exonic
Chromosome Location:   12:16747168-16753711question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000335790 ENST00000428101 ENST00000534484 ENST00000376030 ENST00000441439 ENST00000447609 ENST00000320122 ENST00000540848 ENST00000535535 ENST00000537304 ENST00000534946 ENST00000540445 ENST00000541846 ENST00000539534 ENST00000546281 ENST00000537757 ENST00000546279 ENST00000538051 ENST00000545436 ENST00000540590 ENST00000538020 ENST00000541295 ENST00000261169 ENST00000354662 ENST00000295006 ENST00000433674

Gene Information

The protein encoded by this gene belongs to the rhombotin family of cysteine-rich LIM domain oncogenes. This gene is predominantly expressed in the brain. Related family members LMO1 and LMO2 on chromosome 11 have been reported to be involved in chromosomal translocations in T-cell leukemia. Many alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   LMO3
Gene Name:   LIM domain only 3 (rhombotin-like 2)
Aliases:   DAT1, MGC26081, RBTN3, RBTNL2, RHOM3, Rhom-3
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000048540
Entrez:   55885
Chromosome Mapping:   12p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 34.930000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download