PrimePCR™ SYBR® Green Assay: C6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049341

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: C6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: C6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042078
Assay Design:   exonic
Chromosome Location:   5:41201706-41201806question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000309295 ENST00000568193 ENST00000564962 ENST00000565557 ENST00000565080 ENST00000561589 ENST00000561919 ENST00000567302 ENST00000565268 ENST00000568204 ENST00000564535 ENST00000568089 ENST00000255858 ENST00000540456 ENST00000392772 ENST00000533256 ENST00000530636 ENST00000252015 ENST00000451813 ENST00000432634 ENST00000539834 ENST00000373703 ENST00000357086 ENST00000456211 ENST00000443694 ENST00000302640 ENST00000354582 ENST00000423119 ENST00000534366 ENST00000528919 ENST00000398656 ENST

Gene Information

C6 is a component of complement cascade. It is part of the membrane attack complex which can insert into the cell membrane and cause cell to lyse. People with C6 deficiency are prone to bacterial infection. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   C6
Gene Name:   complement component 6
Aliases:   Not Available
RefSeq:   NC_000005.9 NG_011582.1 NT_006576.16
Ensembl:   ENSG00000039537
Entrez:   729
Chromosome Mapping:   5p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download