PrimePCR™ SYBR® Green Assay: BTK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049227

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BTK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BTK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041964
Assay Design:   exonic
Chromosome Location:   X:100604782-100604890question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000372880 ENST00000308731 ENST00000593634 ENST00000593575

Gene Information

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1 which is an immunodeficiency characterized by the failure to produce mature B lymphocytes and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq Nov 2008]

Gene Symbol:   BTK
Gene Name:   Bruton agammaglobulinemia tyrosine kinase
Aliases:   AGMX1, AT, ATK, BPK, IMD1, MGC126261, MGC126262, PSCTK1, XLA
RefSeq:   NC_000023.10 NG_009616.1 NG_011734.1 NG_012523.1 NT_011651.17
Ensembl:   ENSG00000010671
Entrez:   695
Chromosome Mapping:   Xq21.33-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.240000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download