PrimePCR™ SYBR® Green Assay: DYRK4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049220

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041957
Assay Design:   exonic
Chromosome Location:   12:4721751-4721849question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000542744 ENST00000540757 ENST00000543431 ENST00000544671 ENST00000010132 ENST00000545342

Gene Information

Dual-specificity kinases such as DYRK4 play key roles in cell proliferation survival and development (Zhang et al. 2005 [PubMed 15607427]).[supplied by OMIM Mar 2008]

Gene Symbol:   DYRK4
Gene Name:   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
Aliases:   Not Available
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000010219
Entrez:   8798
UniGene:   Hs.439530
Chromosome Mapping:   12p13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.870000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download