PrimePCR™ SYBR® Green Assay: CYB561, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049208

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: CYB561, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CYB561, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041945
Assay Design:   exonic
Chromosome Location:   17:61511772-61511861question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000392975 ENST00000360793 ENST00000584031 ENST00000581573 ENST00000448884 ENST00000585153 ENST00000582034 ENST00000542042 ENST00000580691 ENST00000582997 ENST00000584291 ENST00000392976 ENST00000409072 ENST00000561234 ENST00000416165 ENST00000560846 ENST00000321844 ENST00000535366 ENST00000372980 ENST00000315005 ENST00000587113 ENST00000426548 ENST00000554473 ENST00000556759 ENST00000555167 ENST00000304613 ENST00000368571 ENST00000368572 ENST00000594987

Gene Information

Description Not Available

Gene Symbol:   CYB561
Gene Name:   cytochrome b-561
Aliases:   FRRS2
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000008283
Entrez:   1534
Chromosome Mapping:   17q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download