This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049206
PrimePCR™ PreAmp for SYBR® Green Assay: NADK, Human
PrimePCR™ Template for SYBR® Green Assay: NADK, Human
NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al. 2001 [PubMed 11594753]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.