PrimePCR™ SYBR® Green Assay: AP2B1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049179

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AP2B1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AP2B1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041916
Assay Design:   exonic
Chromosome Location:   17:33921080-33925346question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000589774 ENST00000262325 ENST00000592545 ENST00000312678 ENST00000589344 ENST00000590432 ENST00000537622 ENST00000593014 ENST00000538556

Gene Information

The protein encoded by this gene is one of two large chain components of the assembly protein complex 2 which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   AP2B1
Gene Name:   adaptor-related protein complex 2, beta 1 subunit
Aliases:   ADTB2, AP105B, AP2-BETA, CLAPB1, DKFZp781K0743
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000006125
Entrez:   163
Chromosome Mapping:   17q11.2-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.770000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download