PrimePCR™ SYBR® Green Assay: MSL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049165

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MSL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MSL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041902
Assay Design:   exonic
Chromosome Location:   X:11778592-11779019question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000312196 ENST00000337339 ENST00000476743 ENST00000421368 ENST00000380693 ENST00000380692 ENST00000398527 ENST00000361672 ENST00000323851 ENST00000414097 ENST00000354944 ENST00000537882

Gene Information

This gene encodes a nuclear protein that is similar to the product of the Drosophila male-specific lethal-3 gene. The Drosophila protein plays a critical role in a dosage-compensation pathway which equalizes X-linked gene expression in males and females. Thus the human protein is thought to play a similar function in chromatin remodeling and transcriptional regulation and it has been found as part of a complex that is responsible for histone H4 lysine-16 acetylation. This gene can undergo X inactivation. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2 7 and 8. [provided by RefSeq Jul 2010]

Gene Symbol:   MSL3
Gene Name:   male-specific lethal 3 homolog (Drosophila)
Aliases:   DKFZp586J1822, MSL3L1
RefSeq:   NC_000023.10 NT_167197.1 NG_012564.1
Ensembl:   ENSG00000005302
Entrez:   10943
Chromosome Mapping:   Xp22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.660000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download