PrimePCR™ SYBR® Green Assay: ARHGAP33, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049148

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGAP33, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARHGAP33, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041885
Assay Design:   exonic
Chromosome Location:   19:36269387-36269488question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000544076 ENST00000375820 ENST00000397198 ENST00000294016 ENST00000373464 ENST00000449886 ENST00000378944 ENST00000314737 ENST00000592958 ENST00000221905 ENST00000587433 ENST00000587447 ENST00000007510 ENST00000286307

Gene Information

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain which is a phosphoinositide binding domain and are involved in intracellular trafficking. Alternative splice variants encoding different isoforms have been identified in this gene. [provided by RefSeq Feb 2010]

Gene Symbol:   ARHGAP33
Gene Name:   Rho GTPase activating protein 33
Aliases:   FLJ39019, NOMA-GAP, SNX26, TCGAP
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000004777
Entrez:   115703
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.040000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download