PrimePCR™ SYBR® Green Assay: TMEM176A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049139

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041876
Assay Design:   exonic
Chromosome Location:   7:150500522-150500824question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000367545 ENST00000448521 ENST00000468694 ENST00000494774 ENST00000432854 ENST00000452661 ENST00000518007 ENST00000237536 ENST00000381603 ENST00000262929 ENST00000370251 ENST00000370249 ENST00000421401 ENST00000440091 ENST00000421422 ENST00000373263 ENST00000540170 ENST00000369833 ENST00000356865 ENST00000545588 ENST00000550078 ENST00000484928 ENST00000004103 ENST00000461345 ENST00000475536 ENST00000468689 ENST00000075120 ENST00000574876 ENST00000572293 ENST00000301396 ENST00000436683 ENST

Gene Information

Description Not Available

Gene Symbol:   TMEM176A
Gene Name:   transmembrane protein 176A
Aliases:   GS188, HCA112
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000002933
Entrez:   55365
UniGene:   Hs.647116
Chromosome Mapping:   7q36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download