This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Same primer pair as used in probe assay qHsaCEP0041840
PrimePCR™ PreAmp for SYBR® Green Assay: RPS17, Human
PrimePCR™ Template for SYBR® Green Assay: RPS17, Human
Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.
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Aurum™ Total RNA Mini KitThe Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials including cultured cells, bacteria, and yeast, as well as animal and plant tissues.
iScript™ Advanced cDNA Synthesis Kit for RT-qPCRThe iScript advanced cDNA synthesis kit for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis by reverse-transcription qPCR.
PrimePCR™ Assays and PanelsExperimentally validated real-time PCR assays are available as individual assays, preplated pathway- and disease-specific panels, or custom- configured plates.
SsoAdvanced™ Universal Real-Time PCR SupermixThe SsoAdvanced™ universal real-time PCR supermixes employ our patented Sso7d fusion enzyme technology for higher processivity, increased PCR inhibitor tolerance, and robust performance with difficult qPCR targets. Available for probe- and dye-based assays in ROX- dependent or ROX-independent real-time PCR detection systems.
Real-Time PCR Detection SystemsReal-time PCR amplification systems combine thermal cyclers with optical reaction modules for singleplex and multiplex detection of fluorophores. All systems feature thermal gradient functionality.
Amplification SoftwareSoftware for Bio-Rad thermal cyclers supports multi-instrument control and collection and analysis of real-time data. Data analysis tools include ΔCq and ΔΔCq analyses and scatter plot, volcano plot, heat map, and clustergram outputs.
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View the products required to complete your real-time PCR experiments.
Pricing for PrimePCR real-time PCR assays is based on assay type, the number of reactions, and plate configuration for 96- and 384-well plates.
Discount applies to bulk orders of the same catalog number and is taken off the list price.
Bulk discounts are shown below; download the pricing flyer for full details.
Real-Time PCR Assays and Controls
5 or more assays
Pathway and Collection Panels
20 or more plates
Custom PlatesCustom 96-well & 384-well plates with 1–96 unique genes
Custom 384-well plates with 97 or more unique genes
30 or more plates
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