PrimePCR™ SYBR® Green Assay: RBMY1A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041834

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041765
Assay Design:   exonic
Chromosome Location:   Y:23710811-23710960question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000328965 ENST00000335071 ENST00000383020 ENST00000382707 ENST00000303902 ENST00000439108 ENST00000382659 ENST00000382673 ENST00000382658 ENST00000382680 ENST00000382677 ENST00000418956 ENST00000303766 ENST00000454978 ENST00000250831 ENST00000414629 ENST00000445779 ENST00000325722 ENST00000373258 ENST00000278816 ENST00000377681 ENST00000489634 ENST00000435364 ENST00000522172 ENST00000229328 ENST00000046640 ENST00000414524

Gene Information

This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine arginine glycine tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3 along with two copies of PTPN13-like Y-linked. [provided by RefSeq Jul 2008]

Gene Symbol:   RBMY1A1
Gene Name:   RNA binding motif protein, Y-linked, family 1, member A1
Aliases:   MGC181956, RBM, RBM1, RBM2, RBMY, RBMY1C, YRRM1, YRRM2
RefSeq:   NC_000024.9 NG_004832.1 NG_012805.1 NT_011875.12
Ensembl:   ENSG00000234414
Entrez:   5940
UniGene:   Hs.380450
Chromosome Mapping:   Yq11.223

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.100000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download