PrimePCR™ SYBR® Green Assay: PRR20C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041810

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRR20C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRR20C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041741
Assay Design:   exonic
Chromosome Location:   13:57729925-57730017question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000360299 ENST00000553116 ENST00000421993 ENST00000457447 ENST00000456194 ENST00000321169 ENST00000273261 ENST00000383703 ENST00000215838 ENST00000405742 ENST00000407817 ENST00000377931 ENST00000377930 ENST00000544357 ENST00000452123 ENST00000434815 ENST00000445351 ENST00000357681 ENST00000341360 ENST00000367218 ENST00000367219 ENST00000391954

Gene Information

This gene is one of five identical loci in a cluster on chromosome 13q21.1. The predicted protein is proline-rich and contains several dopamine D4 receptor signatures and PRINTS domains. [provided by RefSeq Oct 2008]

Gene Symbol:   PRR20C
Gene Name:   proline rich 20C
Aliases:   Not Available
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000229665
Entrez:   729240
Chromosome Mapping:   13q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.950000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download