PrimePCR™ SYBR® Green Assay: DEFA1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041728

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DEFA1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DEFA1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0039006
Assay Design:   exonic
Chromosome Location:   8:6855100-6855234question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000588416 ENST00000534797 ENST00000397615 ENST00000397604 ENST00000529768 ENST00000533410 ENST00000438658 ENST00000354420 ENST00000356187 ENST00000397614 ENST00000334306 ENST00000421030 ENST00000409996 ENST00000395690 ENST00000339553 ENST00000545244 ENST00000454855 ENST00000302118 ENST00000268154 ENST00000370247 ENST00000260502 ENST00000370244 ENST00000370243 ENST00000254528 ENST00000449981 ENST00000327475 ENST00000359357 ENST00000441566 ENST00000297435 ENST00000382692 ENST00000382679 ENST

Gene Information

Description Not Available

Gene Symbol:   DEFA1B
Gene Name:   defensin, alpha 1B
Aliases:   DEFA1, HNP-1, HP-1, HP1
RefSeq:   NC_000008.10 NT_023736.17
Ensembl:   ENSG00000240247
Entrez:   728358
Chromosome Mapping:   8p23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.000000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download