PrimePCR™ SYBR® Green Assay: MAGEA12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041725

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAGEA12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAGEA12, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0039003
Assay Design:   exonic
Chromosome Location:   X:151899885-151900071question
Amplicon Length:   157
Splice Variants Targeted:   ENST00000373491 ENST00000329342 ENST00000370293 ENST00000331220 ENST00000357916 ENST00000393869 ENST00000393900 ENST00000370284 ENST00000543232 ENST00000393876 ENST00000393872 ENST00000370278 ENST00000393902 ENST00000372584 ENST00000372583 ENST00000598245 ENST00000598543 ENST00000594751 ENST00000595895 ENST00000597359 ENST00000595583 ENST00000596509

Gene Information

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders such as dyskeratosis congenita. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Oct 2009]

Gene Symbol:   MAGEA12
Gene Name:   melanoma antigen family A, 12
Aliases:   CT1.12, MAGE12
RefSeq:   NG_013228.1 NC_000023.10 NT_167198.1 NG_015852.1
Ensembl:   ENSG00000213401
Entrez:   4111
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.490000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download