PrimePCR™ SYBR® Green Assay: SCNN1D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041722

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0039000
Assay Design:   exonic
Chromosome Location:   1:1223101-1223195question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000568392 ENST00000570268 ENST00000373857 ENST00000539896 ENST00000353088 ENST00000476071 ENST00000216879 ENST00000350991 ENST00000381658 ENST00000417954 ENST00000528838 ENST00000400780 ENST00000394413 ENST00000508545 ENST00000394414 ENST00000394411 ENST00000336640 ENST00000504198 ENST00000394409 ENST00000356826 ENST00000453001 ENST00000394410 ENST00000308508 ENST00000379116 ENST00000338555 ENST00000325425 ENST00000379099 ENST00000400928 ENST00000373657 ENST00000334463 ENST00000534715 ENST

Gene Information

Description Not Available

Gene Symbol:   SCNN1D
Gene Name:   sodium channel, nonvoltage-gated 1, delta
Aliases:   ENaCd, ENaCdelta, MGC149710, MGC149711, SCNED, dNaCh
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000162572
Entrez:   6339
UniGene:   Hs.512681
Chromosome Mapping:   1p36.3-p36.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download