PrimePCR™ SYBR® Green Assay: SULT1A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041695

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038973
Assay Design:   exonic
Chromosome Location:   16:28604763-28604865question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000358204 ENST00000393481 ENST00000537767 ENST00000335715 ENST00000526384 ENST00000395630 ENST00000350842 ENST00000569554 ENST00000314752 ENST00000566189 ENST00000567512 ENST00000395609 ENST00000395607 ENST00000405237 ENST00000312108 ENST00000395650 ENST00000360719 ENST00000457250 ENST00000440709 ENST00000434492

Gene Information

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones neurotransmitters drugs and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Two alternatively spliced variants that encode the same protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   SULT1A2
Gene Name:   sulfotransferase family, cytosolic, 1A, phenol-preferring, member 2
Aliases:   HAST4, MGC142287, MGC142289, P-PST, ST1A2, STP2, TSPST2
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000197165
Entrez:   6799
UniGene:   Hs.546304
Chromosome Mapping:   16p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.980000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download