PrimePCR™ SYBR® Green Assay: DPPA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041690

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DPPA3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DPPA3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038968
Assay Design:   exonic
Chromosome Location:   12:7864118-7867781question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000262605 ENST00000372904 ENST00000345088 ENST00000419165

Gene Information

This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice this gene may play a role in transcriptional repression cell division and maintenance of cell pluripotentiality. In humans related intronless loci are located on chromosomes 14 and X. [provided by RefSeq Jul 2008]

Gene Symbol:   DPPA3
Gene Name:   developmental pluripotency associated 3
Aliases:   STELLA
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000187569
Entrez:   359787
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.510000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download