PrimePCR™ SYBR® Green Assay: ZNF519, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041677

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF519, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF519, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038955
Assay Design:   exonic
Chromosome Location:   18:14132318-14132407question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000440587 ENST00000373896 ENST00000589498 ENST00000590202 ENST00000592239 ENST00000338702 ENST00000382952 ENST00000290921 ENST00000382895 ENST00000382891 ENST00000382892 ENST00000594419 ENST00000356929 ENST00000601295 ENST00000602023 ENST00000354959 ENST00000599906 ENST00000418100 ENST00000597537 ENST00000336715 ENST00000324048 ENST00000426871 ENST00000368879 ENST00000555447 ENST00000267540 ENST00000571001 ENST00000573408 ENST00000597167

Gene Information

Description Not Available

Gene Symbol:   ZNF519
Gene Name:   zinc finger protein 519
Aliases:   FLJ36809, FLJ42861, HsT2362
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000175322
Entrez:   162655
Chromosome Mapping:   18p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download