PrimePCR™ SYBR® Green Assay: RASSF7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041634

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RASSF7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RASSF7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038912
Assay Design:   exonic
Chromosome Location:   11:561786-562093question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000344375 ENST00000528736 ENST00000454668 ENST00000397583 ENST00000397582 ENST00000431809 ENST00000367838 ENST00000367836 ENST00000367835 ENST00000546300 ENST00000539777 ENST00000537209 ENST00000271357 ENST00000361697 ENST00000381352 ENST00000535907 ENST00000405154 ENST00000542865 ENST00000542004 ENST00000542315 ENST00000541218 ENST00000282884 ENST00000545413 ENST00000541490 ENST00000366788 ENST00000366789 ENST00000534978 ENST00000242108 ENST00000374695

Gene Information

Description Not Available

Gene Symbol:   RASSF7
Gene Name:   Ras association (RalGDS/AF-6) domain family (N-terminal) member 7
Aliases:   C11orf13, HRAS1, HRC1, MGC126069, MGC126070
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000099849
Entrez:   8045
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download