PrimePCR™ SYBR® Green Assay: PCDH11Y, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041633

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038911
Assay Design:   exonic
Chromosome Location:   Y:4925056-4925159question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000333703 ENST00000362095 ENST00000400457 ENST00000215473 ENST00000395337 ENST00000361724 ENST00000373094 ENST00000373097 ENST00000361655 ENST00000406881 ENST00000373088 ENST00000504220 ENST00000298274

Gene Information

This gene belongs to the protocadherin gene family a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 7 cadherin repeats a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene is located in a major X/Y block of homology and its most closely related cadherin superfamily member is located in this homologous region on the X chromosome. The protein is thought to play a fundamental role in cell-cell recognition essential for the segmental development and function of the central nervous system. Transcripts arising from alternative splicing encode isoforms with N- and C-terminal variation. [provided by RefSeq Jul 2008]

Gene Symbol:   PCDH11Y
Gene Name:   protocadherin 11 Y-linked
Aliases:   PCDH-PC, PCDH22, PCDHY
RefSeq:   NC_000024.9 NT_011896.9 NG_011652.1
Ensembl:   ENSG00000099715
Entrez:   83259
UniGene:   Hs.661308
Chromosome Mapping:   Yp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download