PrimePCR™ SYBR® Green Assay: LRRC4C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041576

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038854
Assay Design:   exonic
Chromosome Location:   11:40137768-40137883question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000278198 ENST00000527150 ENST00000528697 ENST00000530763 ENST00000533474

Gene Information

NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818) which is a member of the netrin family of axon guidance molecules (Lin et al. 2003 [PubMed 14595443]).[supplied by OMIM Mar 2008]

Gene Symbol:   LRRC4C
Gene Name:   leucine rich repeat containing 4C
Aliases:   KIAA1580, NGL-1, NGL1
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000148948
Entrez:   57689
UniGene:   Hs.135736
Chromosome Mapping:   11p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.720000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download