This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0041576
PrimePCR™ PreAmp for SYBR® Green Assay: LRRC4C, Human
PrimePCR™ Template for SYBR® Green Assay: LRRC4C, Human
NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818) which is a member of the netrin family of axon guidance molecules (Lin et al. 2003 [PubMed 14595443]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.