PrimePCR™ SYBR® Green Assay: LBH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041529

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038807
Assay Design:   exonic
Chromosome Location:   2:30480310-30480485question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000371627 ENST00000360156 ENST00000354228 ENST00000489216 ENST00000354416 ENST00000343984 ENST00000394610 ENST00000394617 ENST00000394616 ENST00000395323 ENST00000406087 ENST00000401506 ENST00000407930

Gene Information

Description Not Available

Gene Symbol:   LBH
Gene Name:   limb bud and heart development homolog (mouse)
Aliases:   DKFZp566J091, MGC104312, MGC163287
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000213626
Entrez:   81606
UniGene:   Hs.567598
Chromosome Mapping:   2p23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.770000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download