PrimePCR™ SYBR® Green Assay: C1QTNF9B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041525

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: C1QTNF9B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: C1QTNF9B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038803
Assay Design:   exonic
Chromosome Location:   13:24465429-24465526question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000303688 ENST00000383794 ENST00000416649 ENST00000294129 ENST00000413374 ENST00000341520 ENST00000317506 ENST00000319778 ENST00000375110 ENST00000263062 ENST00000341584 ENST00000277541 ENST00000402558 ENST00000379174 ENST00000379166 ENST00000345050 ENST00000377648 ENST00000361874 ENST00000409438 ENST00000409240 ENST00000409868 ENST00000409567 ENST00000394003 ENST00000407639 ENST00000382145 ENST00000382140 ENST00000382137 ENST00000382396 ENST00000397272 ENST00000382071 ENST00000332018 ENST

Gene Information

Description Not Available

Gene Symbol:   C1QTNF9B
Gene Name:   C1QTNF9B
Aliases:   CTRP9B, MGC117159
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000205863
Entrez:   387911
Chromosome Mapping:   13q12.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.520000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download