PrimePCR™ SYBR® Green Assay: ASPDH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041523

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ASPDH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ASPDH, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038801
Assay Design:   exonic
Chromosome Location:   19:51015425-51015541question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000601207 ENST00000389208 ENST00000376916 ENST00000347230 ENST00000555452 ENST00000427522 ENST00000467148 ENST00000398158 ENST00000398165 ENST00000359624 ENST00000352178 ENST00000398168 ENST00000368814 ENST00000274811 ENST00000506378 ENST00000537487 ENST00000251170 ENST00000311565 ENST00000448077 ENST00000360694 ENST00000418635

Gene Information

Description Not Available

Gene Symbol:   ASPDH
Gene Name:   aspartate dehydrogenase domain containing
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000204653
Entrez:   554235
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.800000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download