PrimePCR™ SYBR® Green Assay: SCAMP5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041515

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038793
Assay Design:   exonic
Chromosome Location:   15:75305050-75308955question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000425597 ENST00000562327 ENST00000568018 ENST00000562212 ENST00000567920 ENST00000566872 ENST00000361900 ENST00000568119 ENST00000565989 ENST00000564779 ENST00000545456 ENST00000268099 ENST00000566480 ENST00000565345 ENST00000562363 ENST00000564529 ENST00000389722

Gene Information

Description Not Available

Gene Symbol:   SCAMP5
Gene Name:   secretory carrier membrane protein 5
Aliases:   DKFZp686L1799, MGC24969
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000198794
Entrez:   192683
UniGene:   Hs.374180
Chromosome Mapping:   15q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download