PrimePCR™ SYBR® Green Assay: MDM4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041514

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MDM4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MDM4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038792
Assay Design:   exonic
Chromosome Location:   1:204494629-204495489question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000330710 ENST00000409195 ENST00000409273 ENST00000295237 ENST00000367182 ENST00000367180 ENST00000507825 ENST00000454264 ENST00000367183 ENST00000391947

Gene Information

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity and have been shown to be overexpressed in a variety of human cancers. However unlike MDM2 which degrades p53 this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   MDM4
Gene Name:   Mdm4 p53 binding protein homolog (mouse)
Aliases:   DKFZp781B1423, HDMX, MDMX, MGC132766, MRP1
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000198625
Entrez:   4194
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.890000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download