PrimePCR™ SYBR® Green Assay: RP11-347C12.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041512

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-347C12.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-347C12.1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038790
Assay Design:   exonic
Chromosome Location:   16:30234651-30234740question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000435324 ENST00000550983 ENST00000357796 ENST00000424293 ENST00000524087 ENST00000532298 ENST00000517539 ENST00000424340 ENST00000520915 ENST00000543463 ENST00000354563 ENST00000533640 ENST00000532254 ENST00000448012 ENST00000458643 ENST00000542817 ENST00000415645 ENST00000357370 ENST00000451409 ENST00000537951 ENST00000429990 ENST00000399147 ENST00000355290 ENST00000529716 ENST00000452313

Gene Information

Description Not Available

Gene Symbol:   RP11-347C12.1
Gene Name:   Putative NPIP-like protein LOC613037
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000198064
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.880000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download