PrimePCR™ SYBR® Green Assay: ZNF595, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041510

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF595, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF595, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038788
Assay Design:   exonic
Chromosome Location:   4:53232-53337question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000421367 ENST00000438123 ENST00000539605 ENST00000490531 ENST00000432854 ENST00000600293 ENST00000318003 ENST00000589606 ENST00000256458 ENST00000263253 ENST00000411486 ENST00000432087 ENST00000441687 ENST00000509152 ENST00000526473 ENST00000325147 ENST00000382762 ENST00000590550 ENST00000257261 ENST00000522056 ENST00000278840 ENST00000405198 ENST00000317368 ENST00000357586 ENST00000356015 ENST00000432560 ENST00000531380 ENST00000352410 ENST00000566377 ENST00000598234 ENST00000314529 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF595
Gene Name:   zinc finger protein 595
Aliases:   FLJ31740
RefSeq:   NC_000004.11 NT_037622.5
Ensembl:   ENSG00000197701
Entrez:   152687
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download