PrimePCR™ SYBR® Green Assay: LCN12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041493

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038771
Assay Design:   exonic
Chromosome Location:   9:139846784-139846892question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000320736 ENST00000445522 ENST00000447234 ENST00000373164 ENST00000244565 ENST00000371633 ENST00000300591 ENST00000579038 ENST00000582408 ENST00000441551 ENST00000398722 ENST00000536736 ENST00000441893 ENST00000360890 ENST00000325239 ENST00000413659 ENST00000348624 ENST00000360614 ENST00000585374 ENST00000593119 ENST00000540670 ENST00000590729 ENST00000331569 ENST00000536441 ENST00000265641 ENST00000372437 ENST00000372408 ENST00000457953 ENST00000372421 ENST00000404771 ENST00000372440 ENST

Gene Information

Members of the lipocalin family such as LCN12 have a common structure consisting of an 8-stranded antiparallel beta-barrel that forms a cup-shaped ligand-binding pocket or calyx. Lipocalins generally bind small hydrophobic ligands and transport them to specific cells (Suzuki et al. 2004 [PubMed 15363845]).[supplied by OMIM Aug 2009]

Gene Symbol:   LCN12
Gene Name:   lipocalin 12
Aliases:   MGC34753, MGC48935
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000184925
Entrez:   286256
UniGene:   Hs.440519
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download