PrimePCR™ SYBR® Green Assay: POLD4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041481

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: POLD4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POLD4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038759
Assay Design:   exonic
Chromosome Location:   11:67119124-67119227question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000383389 ENST00000422919 ENST00000510769 ENST00000383366 ENST00000508196 ENST00000429253 ENST00000412440 ENST00000312419 ENST00000539074 ENST00000329008 ENST00000395275 ENST00000395276 ENST00000431984 ENST00000446128 ENST00000412588 ENST00000405452 ENST00000546424 ENST00000452298 ENST00000449876 ENST00000598251

Gene Information

This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Mar 2012]

Gene Symbol:   POLD4
Gene Name:   polymerase (DNA-directed), delta 4
Aliases:   POLDS, p12
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000175482
Entrez:   57804
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.350000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download