PrimePCR™ SYBR® Green Assay: KCNK7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041475

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038753
Assay Design:   exonic
Chromosome Location:   11:65360504-65360615question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000525254 ENST00000530380 ENST00000340313 ENST00000394216 ENST00000394217 ENST00000342202 ENST00000393359 ENST00000341184 ENST00000315266 ENST00000478722 ENST00000543237 ENST00000305960 ENST00000369774 ENST00000355946 ENST00000377306 ENST00000451767 ENST00000277865 ENST00000537649 ENST00000373886 ENST00000319004 ENST00000576778 ENST00000520356 ENST00000520727 ENST00000429442 ENST00000377838 ENST00000520214 ENST00000523399 ENST00000243300 ENST00000428173

Gene Information

This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel; however it may require other non-pore-forming proteins for activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNK7
Gene Name:   potassium channel, subfamily K, member 7
Aliases:   K2p7.1, MGC118782, MGC118784, TWIK3
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000173338
Entrez:   10089
UniGene:   Hs.175218
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.200000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download