PrimePCR™ SYBR® Green Assay: ZNF680, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041474

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038752
Assay Design:   exonic
Chromosome Location:   7:64004085-64004699question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000309683 ENST00000447137 ENST00000476120 ENST00000545510 ENST00000319636 ENST00000282869 ENST00000328747 ENST00000450302 ENST00000591366 ENST00000588146 ENST00000592160 ENST00000343769 ENST00000595282 ENST00000597083 ENST00000335117 ENST00000427401 ENST00000594419 ENST00000596797 ENST00000601440 ENST00000291750 ENST00000261560 ENST00000599548 ENST00000594110 ENST00000595401 ENST00000600692 ENST00000599296 ENST00000311048 ENST00000598331 ENST00000594363 ENST00000594947 ENST00000600162 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF680
Gene Name:   zinc finger protein 680
Aliases:   FLJ90430
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000173041
Entrez:   340252
UniGene:   Hs.520886
Chromosome Mapping:   7q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 37.450000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download