PrimePCR™ SYBR® Green Assay: OBP2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041469

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: OBP2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: OBP2B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038747
Assay Design:   exonic
Chromosome Location:   9:136080700-136080797question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000326261 ENST00000538872 ENST00000340780 ENST00000342114 ENST00000371776 ENST00000539850 ENST00000412584 ENST00000449022 ENST00000397721 ENST00000334010 ENST00000313070 ENST00000372034 ENST00000372032 ENST00000228506 ENST00000301744 ENST00000458723 ENST00000278071 ENST00000333850 ENST00000260947 ENST00000397910 ENST00000562137 ENST00000568103 ENST00000575724

Gene Information

Description Not Available

Gene Symbol:   OBP2B
Gene Name:   odorant binding protein 2B
Aliases:   MGC119022, hOBPIIb
RefSeq:   NC_000009.11 NT_035014.4 NW_003315925.1
Ensembl:   ENSG00000171102
Entrez:   29989
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.900000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download