PrimePCR™ SYBR® Green Assay: RPL29, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041455

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038733
Assay Design:   exonic
Chromosome Location:   3:52028017-52028133question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000520584 ENST00000389301 ENST00000568369 ENST00000567457 ENST00000426981 ENST00000294189 ENST00000466397 ENST00000479017 ENST00000495383 ENST00000475248 ENST00000492277

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq Jul 2008]

Gene Symbol:   RPL29
Gene Name:   ribosomal protein L29
Aliases:   HIP, HUMRPL29, L29, MGC88589
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000162244
Entrez:   6159
UniGene:   Hs.425125
Chromosome Mapping:   3p21.3-p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 35.550000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download