PrimePCR™ SYBR® Green Assay: LY6E, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041450

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038728
Assay Design:   exonic
Chromosome Location:   8:144102759-144102848question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000016171 ENST00000537011 ENST00000438749 ENST00000333756 ENST00000414786 ENST00000445220 ENST00000394697 ENST00000566433 ENST00000254235 ENST00000538642 ENST00000537579 ENST00000302538 ENST00000544583 ENST00000517503 ENST00000292494 ENST00000429120 ENST00000521699 ENST00000520531 ENST00000520466 ENST00000521003 ENST00000522528 ENST00000522971 ENST00000519611 ENST00000521182 ENST00000519546 ENST00000523847 ENST00000522024

Gene Information

Description Not Available

Gene Symbol:   LY6E
Gene Name:   lymphocyte antigen 6 complex, locus E
Aliases:   RIG-E, RIGE, SCA-2, SCA2, TSA-1
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000160932
Entrez:   4061
UniGene:   Hs.521903
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.960000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download