PrimePCR™ SYBR® Green Assay: NBL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041445

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NBL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NBL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038723
Assay Design:   exonic
Chromosome Location:   1:19981531-19981650question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000323327 ENST00000587084 ENST00000602662 ENST00000602293 ENST00000428975 ENST00000289749 ENST00000451758 ENST00000439664 ENST00000375136 ENST00000425400 ENST00000439278 ENST00000427894 ENST00000548815

Gene Information

This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream chromosome 1 open reading frame 151 locus (GeneID 440574) have been observed.[provided by RefSeq Feb 2011]

Gene Symbol:   NBL1
Gene Name:   neuroblastoma, suppression of tumorigenicity 1
Aliases:   D1S1733E, DAN, DAND1, MGC8972, NB, NO3
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000158747
Entrez:   4681
Chromosome Mapping:   1p36.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.470000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download