PrimePCR™ SYBR® Green Assay: RPUSD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041442

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RPUSD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RPUSD3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038720
Assay Design:   exonic
Chromosome Location:   3:9883667-9883889question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000433555 ENST00000433972 ENST00000418713 ENST00000427174 ENST00000424438 ENST00000383820 ENST00000433535 ENST00000393847 ENST00000572888 ENST00000412757 ENST00000328300 ENST00000361603 ENST00000365713 ENST00000371628 ENST00000439603 ENST00000304778 ENST00000422289

Gene Information

Description Not Available

Gene Symbol:   RPUSD3
Gene Name:   RNA pseudouridylate synthase domain containing 3
Aliases:   FLJ34707, FLJ37268, MGC29784
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000156990
Entrez:   285367
Chromosome Mapping:   3p25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.993000
y-intercept 34.690000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download