PrimePCR™ SYBR® Green Assay: POGZ, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041428

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038706
Assay Design:   exonic
Chromosome Location:   1:151403214-151413408question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000475231 ENST00000463781 ENST00000573208 ENST00000302102 ENST00000545399 ENST00000543770 ENST00000602133 ENST00000423902 ENST00000373233 ENST00000373624 ENST00000373620 ENST00000394219 ENST00000394215 ENST00000443842 ENST00000599531 ENST00000392723 ENST00000271715 ENST00000409503 ENST00000531094 ENST00000491586 ENST00000533461 ENST00000533351 ENST00000450842 ENST00000361398 ENST00000540984 ENST00000351288 ENST00000439886 ENST00000409031 ENST00000431183 ENST00000453750 ENST00000376398

Gene Information

The protein encoded by this gene appears to be a zinc finger protein containing a transposase domain at the C-terminus. This protein was found to interact with the transcription factor SP1 in a yeast two-hybrid system. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq Aug 2010]

Gene Symbol:   POGZ
Gene Name:   pogo transposable element with ZNF domain
Aliases:   KIAA0461, MGC71543, SUHW5, ZNF280E, ZNF635, ZNF635m
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143442
Entrez:   23126
UniGene:   Hs.489873
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.730000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download